TABLE 2.
IC50 values (concentrations causing 50% growth inhibition of the pathogen), MIC values, and MBC values obtained in two individual screenings: the first screening starting at 64μM, the second screening starting at 32 μMa
| IC50 (μM) |
MIC (μM) |
MBC (μM) |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMP | Repeat | MRC5-SV2 | S. aureusATCC 6538 | E. coliATCC 8739 | P. aeruginosaATCC 9027 |
C. albicans B59630 |
A. fumigatusB42928 | S. aureus ATCC 6538 | E. coliATCC 8739 | P. aeruginosaATCC 9027 | S. aureusATCC 6538 | E. coliATCC 8739 | P. aeruginosaATCC 9027 |
| Hill-Knot2 | 1 | 9.73 | >64.00 | >64.00 | >64.00 | >64.00 | >64.00 | >64.00 | >64.00 | >64.00 | |||
| 2 | 3.76 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | ||||
| Hill-Knot3 | 1 | >64.00 | >64.00 | >64.00 | 22.63 | >64.00 | >64.00 | >64.00 | >64.00 | >64.00 | |||
| 2 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | ||||
| Hill-Def2a | 1 | >64.00 | <0.25 | 35.33 | 32.86 | >64.00 | >64.00 | 0.25 | >64.00 | >64.00 | static | ||
| 2 | >32.00 | 0.19 | >32.00 | >32.00 | >32.00 | >32.00 | 2.00 | >32.00 | >32.00 | static | |||
| Hill-Def4 | 1 | >64.00 | 0.50 | >64.00 | 32.00 | >64.00 | >64.00 | 1.00 | >64.00 | >64.00 | static | ||
| 2 | >32.00 | 0.70 | >32.00 | >32.00 | >32.00 | >32.00 | 8.00 | >32.00 | >32.00 | static | |||
| Hill-Def2b | 1 | >64.00 | 8.50 | >64.00 | >64.00 | >64.00 | >64.00 | 64.00 | 32.00 | >64.00 | static | ||
| 2 | >32.00 | 13.45 | >32.00 | >32.00 | >32.00 | >32.00 | 32.00 | 64.00 | >32.00 | static | |||
| Hill-Cec1 | 1 | >64.00 | >64.00 | <0.25 | <0.25 | >64.00 | >64.00 | >64.00 | 0.25 | 1.00 | 1.00 | 4.00 | |
| 2 | >32.00 | >32.00 | 0.21 | 0.20 | >32.00 | >32.00 | >32.00 | 0.50 | 0.50 | 0.50 | 0.50 | ||
| Hill-Cec2 | 1 | >64.00 | >64.00 | 0.50 | 0.93 | >64.00 | >64.00 | >64.00 | 1.00 | 4.00 | 1.00 | 16.00 | |
| 2 | >32.00 | 4.36 | 0.31 | 0.76 | >32.00 | >32.00 | >32.00 | 2.00 | 2.00 | 2.00 | 2.00 | ||
| Hill-Cec3 | 1 | >64.00 | >64.00 | 0.49 | 0.40 | >64.00 | >64.00 | >64.00 | 1.00 | 4.00 | 1.00 | 16.00 | |
| 2 | >32.00 | >32.00 | 0.22 | 0.79 | >32.00 | >32.00 | >32.00 | 0.50 | 2.00 | 32.00 | 2.00 | ||
| Hill-Cec4 | 1 | 37.37 | >64.00 | 0.50 | <0.25 | >64.00 | >64.00 | >64.00 | 1.00 | 1.00 | static | 16.00 | |
| 2 | >32.00 | >32.00 | 0.32 | 0.22 | >32.00 | >32.00 | >32.00 | 2.00 | 2.00 | 2.00 | 2.00 | ||
| Hill-Cec5 | 1 | >64.00 | >64.00 | 0.50 | 2.61 | >64.00 | >64.00 | >64.00 | 1.00 | 16.00 | static | static | |
| 2 | >32.00 | >32.00 | 0.23 | 3.60 | >32.00 | >32.00 | >32.00 | 0.50 | 32.00 | 0.50 | 32.00 | ||
| Hill-Cec7 | 1 | >64.00 | >64.00 | 0.38 | 0.40 | >64.00 | >64.00 | >64.00 | 1.00 | 4.00 | 1.00 | 16.00 | |
| 2 | >32.00 | >32.00 | 0.32 | 0.78 | >32.00 | >32.00 | >32.00 | 2.00 | 2.00 | 2.00 | 2.00 | ||
| Hill-Cec8 | 1 | >64.00 | >64.00 | 0.45 | 1.29 | >64.00 | >64.00 | >64.00 | 1.00 | 4.00 | 1.00 | 64.00 | |
| 2 | >32.00 | >32.00 | 0.68 | 1.73 | >32.00 | >32.00 | >32.00 | 2.00 | 8.00 | 8.00 | 8.00 | ||
| Hill-Cec9 | 1 | >64.00 | >64.00 | 0.43 | 0.38 | >64.00 | >64.00 | >64.00 | 1.00 | 1.00 | 1.00 | static | |
| 2 | >32.00 | >32.00 | 0.21 | 0.88 | >32.00 | >32.00 | >32.00 | 0.50 | 2.00 | 0.50 | 2.00 | ||
| Hill-Cec10 | 1 | >64.00 | 32.74 | <0.25 | <0.25 | >64.00 | >64.00 | >64.00 | 0.25 | 1.00 | 0.25 | 16.00 | |
| 2 | >32.00 | >32.00 | 0.22 | 0.22 | >32.00 | >32.00 | >32.00 | 0.50 | 2.00 | 32.00 | 2.00 | ||
| Hill-Cec11 | 1 | >64.00 | >64.00 | <0.25 | <0.25 | >64.00 | >64.00 | >64.00 | 1.00 | 1.00 | 1.00 | 4.00 | |
| 2 | >32.00 | >32.00 | 0.22 | 0.24 | >32.00 | >32.00 | >32.00 | 0.50 | 2.00 | 32.00 | 8.00 | ||
| Hill-Cec12 | 1 | >64.00 | >64.00 | <0.25 | <0.25 | >64.00 | >64.00 | >64.00 | 0.25 | 1.00 | 1.00 | static | |
| 2 | 19.45 | >32.00 | 0.32 | 0.23 | >32.00 | >32.00 | >32.00 | 2.00 | 2.00 | 8.00 | 8.00 | ||
| Hill-Cec13 | 1 | >64.00 | >64.00 | <0.25 | <0.25 | >64.00 | >64.00 | >64.00 | 1.00 | 1.00 | 1.00 | 64.00 | |
| 2 | >32.00 | >32.00 | 0.23 | 0.25 | >32.00 | >32.00 | >32.00 | 0.50 | 2.00 | 0.50 | 2.00 | ||
| Hill-Stom1 | 1 | 11.65 | 58.58 | >64.00 | 16.00 | >64.00 | >64.00 | >64.00 | >64.00 | >64.00 | |||
| 2 | 24.42 | >32.00 | >32.00 | 17.67 | >32.00 | >32.00 | >32.00 | >32.00 | >32.00 | ||||
| Hill-Dip6 | 1 | 4.54 | >32.00 | 0.85 | >32.00 | >64.00 | >64.00 | >64.00 | 2.00 | >32.00 | static | ||
| 2 | 7.73 | >32.00 | 1.00 | >32.00 | >32.00 | >32.00 | >32.00 | 2.00 | >64.00 | 8.00 | |||
a
Peptides with activity < 32 μM against either the cell line (MRC5-SV2) or the microbial reference strains are shown. Static, bacteriostatic activity. Values in bold text indicate that antimicrobial activity could be measured within the tested concentration ranges for the AMP.