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. 2021 Dec 16;135(5):jcs258964. doi: 10.1242/jcs.258964

Fig. 1.

Fig. 1.

Insulin stimulates FA uptake and transport in endothelium indirectly by signaling to adipocytes. (A) Diagram showing the experimental workflow. (B) Conditioned media produced by adipocytes in the presence of increasing concentrations of insulin (ICM) stimulate endothelial FA uptake. DMEM+I, non-conditioned media with equivalent concentration of added insulin. (C) Conditioned media produced by incubating adipocytes with 0 (CM) or 20 nM insulin (ICM) for the indicated times stimulate endothelial FA uptake. (D) ICM maximally induced endothelial cell FA uptake within 30 min of incubation upon ECs. In B–D, data are mean±s.e.m, n=3 biological replicates for every condition. Each experiment was replicated at least twice. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 for ICM; #P<0.05 and ##P<0.01 for CM (one-way ANOVA with Dunnett's test for multiple comparisons). Statistical comparisons are relative to (B) the 0 nM insulin condition, (C) DMEM+I at time zero, and (D) DMEM+I at the corresponding times. AU, arbitrary units; RFU, relative fluorescence units.