Fig. 3.

The capacity of ICM to induce endothelial FA uptake is dependent on its lactate concentration. (A) Heatmap of metabolite ratios for ICM versus CM over the course of 30 h. The data are represented in terms of log₂ of the fold change (FC). n=4 biological replicates for the 1–12 h timepoints, and n=6 for 24 h and 30 h timepoints. (B) Lactate concentration in CM and ICM at the indicated times. n=4 biological replicates for the 1–12 h timepoints, and n=6 for 24 h and 30 h timepoints. (C) Lactate concentration in CM and ICM after treatment with the LDHA inhibitor GSK2837808A at the indicated concentrations. n=2 or 3 biological replicates per condition. (D) ICM from adipocytes treated with GSK2837808A exhibit reduced capacity to induce endothelial FA uptake. n=3 biological replicates per condition. Data in B–D are mean±s.e.m. Each experiment was replicated at least twice. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 for CM compared to time zero in B and for ICM compared to CM in C,D; ^#P<0.05 and ^####P<0.0001 for ICM compared to time zero in B (one-way ANOVA with Dunnett's test for multiple comparisons). RFU, relative fluorescence units.