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. 2021 Dec 15;134(24):jcs258864. doi: 10.1242/jcs.258864

Fig. 6.

Fig. 6.

Fz3-induced Vangl2 phosphorylation is necessary for the inhibition of the Vangl2–Pk3 interaction. (A) Scheme of Vangl2 constructs with alanine substitutions. Conserved serine and threonine (S/T) clusters I and II are indicated. (B,C) Effect of Fz3 on the biotinylation of various Vangl2 constructs by BLN–Pk3. Embryos were injected dorsoanimally with mRNAs encoding Vangl2 constructs (50 pg each), BLN–Pk3 (200 pg), or Fz3–FLAG mRNA (200 pg or 400 pg) (B) and 400 pg (C); and collected at stage 13. (B) The Vangl2-2A mutant is partly resistant to the Fz3 inhibitory effect. (C) Vangl2-7A is completely resistant to Fz3 and is not upshifted (black and white arrowheads). Immunoblotting (IB) with anti-biotin, anti-HA and anti-FLAG antibodies was done as indicated. Asterisks represent a non-specific band. Data are representative of three experiments.