Fig. 2.

Nup160 DNA-PAINT egg chamber imaging. (A) An illustration of the position of the focal plane imaged in (B), which is 15 to 25 µm above the coverslip and inside the egg chamber. (B) DNA-PAINT image showing nurse cell and follicle cell nuclei. (C,D) A magnified view of the area outlined by the red box in B showing a cross-sectional view of NPCs in the nuclear membrane (C). The intensity distribution between the outer and inner rings of the NPCs in region in C is shown in D, with the distance measured as 60 nm. (E,F) Detailed views of NPCs in the follicle cell nuclear membrane (E) and the nurse cell nuclear membrane (F), showing distinct ring structures with hollow centres. (G,H) Detailed views of NPCs from E and F fitted with a circle and diameters of 96.3 and 92.9 respectively. Data shown are representative of 27 images. Scale bars: 1 µm (A); 500 nm (C,E,F).