Fig. 5.

Deleting the VBD in VEC-αC, but not replacing it enhances detergent extractability. (A) To separate the Triton-soluble (sol) from the Triton-insoluble (is) fraction of the various forms of VE-cadherin, confluent MDMVECs were incubated with Triton buffer for 10 min (soluble fraction), followed by lysing the residual monolayers in a harsh SDS-containing lysis buffer (insoluble fraction). Both fractions were subjected to western blotting for the indicated antigens. (B) Quantification of soluble/insoluble fractions from four independent experiments. Results are shown as mean±s.e.m. *P≤0.05; **P≤0.01; ***P≤0.001 (one-way ANOVA with Tukey's multiple comparisons test).