Figure 3.

Highly correlated proteins and individual absolute protein levels for four uEV marker proteins in individual rats showed almost perfect correlations. (A–F): control diet; (G –L): high K⁺ diet. R denotes Pearson’s correlation coefficient, the blue dashed diagonal denotes perfect positive correlation, a single asterisk (*) denotes 0.01<P<0.05; a double asterisk (**) denotes 0.001<P<0.01; a triple asterisk (***) denotes 0.0001<P<0.001; and a quadruple asterisk (****) denotes P<0.0001. Although some proteins showed P<0.05, they still fulfilled the stringent criteria we set (except Cd9, see text). All axes denote normalized log₁₀ iBAQ percentages for uEV or kidney. Cd9, an established marker protein for uEVs, had greater (100-fold) protein abundance in uEVs than in kidney.