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HCOV-229E infects hKEpC and kSPH. (A) hKEpC and kSPH express CD13 (ANPEP), which is the receptor for HCOV-229E. (B) CD13 immunostaining of adult kidney tissue, hKEpC, kSPH, and the in vivo tubuloids derived thereof. (C) After infection with HCOV-229E, hKEpC and kSPH express the viral RNA at similar levels as human lung cells (hLung). EMA, Epithelial Membrane Antigen; DAPI, 4′,6-diamidi no-2-phenylindole. (Scale bar: B: 100μm).
