Table 2.
Cell Types Used in Porous Substrate Systems
| Type | Organism | Cell Line | Description | Surface Coating | Viability | Transfection Efficiency | Ref. |
|---|---|---|---|---|---|---|---|
| Immortal | Human | A375 | Melanoma | >90% | >90% | [168] | |
| BEAS-2B | Transformed Bronchial Epithelial | Gelatin, BSA, PEG | [168] | ||||
| HEK293 | Embryonic Kidney | Poly-L-Lysine or Fibronectin | >90% | 80% | [151] | ||
| HeLa | Cervical Epithelial | Poly-L-Lysine [151] or Fibronectin [151, 154] | >95% [151] | >80% [151] | [151, 153, 154, 159] | ||
| HL-60 | Promyeloblast, Suspended | >90% | 65%* | [155] | |||
| HT1080 | Connective Tissue | Fibronectin [152] | 50% [153] | [152, 153, 159] | |||
| Jurkat | T Lymphocyte, Suspended | Poly-L-Lysine or Fibronectin [151], PEG-Silane [163] | >95% [151] | 75% [151] | [151, 163] | ||
| KG1a | Promyeloblast, Suspended | PEG-Silane | 96% | [163] | |||
| K562 | Lymphoblast, Suspended | PEG-Silane [163] | 92% [163] | 83.4% [163] | [163, 164] | ||
| MDA-MB231 | Mammary Epithelial | Fibronectin | >99% [152] | 70% [152] | [152, 158] | ||
| NK-92 | Natural Killer, Suspended | 90% | 74% | [165] | |||
| Mouse | NIH3T3 | Embryonic Fibroblast | Poly-L-Lysine or Fibronectin [151] | >95% [151] | 75% [151] | [151, 156] | |
| Rat | H9C2 | Embryonic Cardio-myoblast | [160, 165, 166] | ||||
| Primary | Human | HCF-a | Myofibroblast | 95.6% | 90.5% | [168] | |
| None | Leukocyte, Suspended | PEG-Silane | [163] | ||||
| Mouse | None | Cardiomyocyte | 86% | 86% | [161] | ||
| None | Embryonic Fibroblast | >90% [161] | >90% [161] | [160, 161] | |||
| None | Neuron | Poly-D-Lysine | >90% | [153] | |||
| Stem | Human | GBM157 | Glioma Stem | >70% | [162] | ||
| GBM528 | Glioma Stem | [162] | |||||
| Mouse | CCE | Embryonic Stem | Gelatin [167] | >85% [167] | [157, 167] |
*
Delivery efficiency, not transfection efficiency. No nucleic acids were delivered in this study.