Table 4.
Mean oocyst counts from DMFAs in An. farauti according to RDT, microscopy and qPCR.
| RDT, microscopy & qPCR results | Proportion DMFAs resulting in mosquito infection | Proportion of mosquitoes infected* | Oocyst number | ||||
|---|---|---|---|---|---|---|---|
| n/N | % (95% CI) | n/N | % (95% CI) | average (range) | |||
| RDT | |||||||
| HRP2 | 15/55 | 27.3 (15.5 - 39.1)a |
564/966 | 58.4 (55.2 - 61.5) |
6 (1-106) |
||
| pLDH | 13/37 | 35.1 (19.7 - 50.5)b |
349/863 | 40.4 (37.2 - 43.8) |
27 (1-534) |
||
| HRP2 & pLDH | 10/90 | 11.1 (4.6 - 17.6)c |
60/415 | 14.5 (11.2 - 18.2) |
3 (1-17) |
||
| Microscopy | |||||||
| Pf asexual only | 7/68 | 10.3 (3.1 - 17.6)d |
66/376 | 17.6 (13.8 - 21.8) |
5 (1-16) |
||
| Pf. + gametocytes | 3/20 | 15 (0 - 30.6 § ) |
47/94 | 50.0 (39.5 - 60.5) |
3 (1-9) |
||
| Pv. asexual only | 9/28 | 32.1 (14.8 - 49.3)e |
218/627 | 34.8 (31 - 38.6) |
9 (1-93) |
||
| Pv. + gametocytes | 14/24 | 58.3 (38.6 - 78)f |
424/749 | 55.9 (52.8 - 60.2) |
19 (1-534) |
||
| Pf. + gametocytes & Pv + gametocytes | 2/2 | 100 (NA) |
13/69 | 18.8 (10.4 - 30.1) |
3 (1-13) |
||
| Pf. asexual only & Pv gametocytes | 1/1 | 100 (NA) |
83/89 | 93.3 (85.9 - 97.5) |
7 (1-36) |
||
| Microscopy Negative | 2/39 | 5.1 (0 - 12 § ) |
13/38 | 34.2 (19.6 - 51.4) |
9 (1-29) |
||
| qPCR | |||||||
| P. falciparum | 8/80 | 10 (3.4 - 16.6) |
135/481 | 28 (24.1 - 32.3) |
3 (1-43) |
||
| Pv. | 24/55 | 43.6 (30.5 - 56.7) |
735/1501 | 49 (46.4 - 51.5) |
12 (1-534) |
||
| P. falciaprum & P. vivax | 4/20 | 20 (2.5 - 37.5) |
96/248 | 38.7 (32.6 - 45.1) |
8 (1-106) |
||
| qPCR Negative | 2/27 | 7.4 (0 - 17.3) § |
7/14 | 50.0 (23.0 – 77) |
7 (1-24) |
||
Results of the diagnosis by RDT, microscopy and qPCR, N =182 while n = 38 in the successful infections.
*only infected mosquitoes were considered (i.e., uninfected mosquitoes were not included into this calculation); significant differences were observed in the proportions a vs. c; b vs. c, d vs. e and d vs. f. No significant difference was observed between a vs. b, p=0.43.
§95% confidence interval includes negative values.
All samples were collected from symptomatic RDT positive patients. Values are presented either as proportion (n/N) and percent, or as average and minimum to maximum range.
Pf., P. falciparum; Pv., P. vivax.