KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Asc (AL177) antibody | AdipoGen | Cat# AG-25B-0006; RRID: AB_2490440 |
| Rat anti-Caspase-1 (clone 4B4) antibody | Genentech | Cat# CASP-1(mu):4175 |
| Rabbit anti-GAPDH antibody | Sigma-Aldrich | Cat# G9545; RRID: AB_796208 |
| Rabbit anti-GSDMD antibody [EPR19828] | Abcam | Cat# ab209845; RRID: AB_2783550 |
| Mouse anti-NLRP3/NALP3 (Cryo-2) antibody | AdipoGen | Cat# AG-20B-0014-C100; RRID: AB_2490202 |
| APC rat anti-mouse/human CD11b antibody | Bio Legend | Cat# 101212; RRID: AB_312795 |
| Mouse specific rabbit anti-ASC/TMS1 (D2W8U) antibody | Cell Signaling Technology | Cat# 67824; RRID: AB_2799736 |
| Rabbit anti-β-actin antibody | Cell Signaling Technology | Cat# 4967; RRID: AB_330288 |
| Goat anti-rabbit-AlexaFluor488 (H+L) antibody | Thermo Fisher Scientific | Cat# A-11008, RRID: AB_143165 |
| Goat anti-rat-AlexaFluor594 antibody | Invitrogen | Cat# A11007; RRID: AB_141374 |
| Rabbit anti-IL-1β antibody | GeneTex | Cat# GTX74034; RRID: AB_378141 |
| IRDye® 680LT donkey anti-mouse IgG (H+L) antibody | LI-COR Biotechnology | Cat# 926-68022; RRID: AB_10715072 |
| IRDye® 800CW goat anti-rat IgG (H+L) antibody | LI-COR Biotechnology | Cat# 926-32219; RRID: AB_1850025 |
| Mouse anti-Aβ (82E1) antibody | IBL America | Cat# 10323; RRID: AB_1630806 |
| Mouse IgG Isotype Control antibody | Thermo Fisher Scientific | Cat# 10400C; RRID: AB_2532980 |
| Mouse TLR2 neutralizing antibody (C9A12) | InvivoGen | Cat# mabg-mtlr2; RRID: AB_11125339 |
| Mouse TLR5 neutralizing antibody (Q23D11) | InvivoGen | Cat# mabg-mtlr5; RRID: AB_11124926 |
| Rat anti-mouse CD11b antibody | Serotec by Bio-Rad | Cat# MCA711; RRID: AB_321292 |
| Rat IgG Isotype Control antibody | Thermo Fisher Scientific | Cat# 10700; RRID: AB_2610661 |
| TLR4/MD-2 Complex Monoclonal Antibody (MTS510) | Invitrogen | Cat# 14-9924-82; RRID: AB_468617 |
| Goat anti-Trem2 antibody | GeneTex | Cat# GTX47596; RRID: AB_10618011 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Ampicillin sodium salt | Carl Roth | Cat# K029.1 |
| 4’,6-Diamidino-2′-phenylindol-dihydrochloride (DAPI) | Thermo Fisher Scientific | Cat# 62247 |
| Adenosine 5′-triphosphate disodium salt hydrate | Sigma-Aldrich | Cat# A2383 |
| Amyloid β-Protein (1-42) (HFIP-treated) | Bachem AG | Cat# 4090148 |
| Bovine Serum Albumin - Fraction V | Rockland Immunochemicals, Inc. | Cat# BSA-1000 |
| FAM-labeled Amyloid β | Peptide Specialty Laboratories GmbH (PSL) | ID# CEM112812FL |
| Fetal Bovine Serum | LIFE Technologies | Cat# 10270106 |
| Guanidine hydrochloride | Carl Roth | Cat# 6069.3 |
| HisTrap™ FF crude | GE Healthcare | Cat# 17528601 |
| IFM-2384 | IFM Therapeutics | gift from IFM |
| Lipopolysaccharide from Escherichia coli K12 | InvivoGen | Cat# tlrl-eklps |
| MCC950 (CRID3) | InvivoGen | Cat# inh-mcc |
| N2-Supplement | GIBCO by Thermo Fisher Scientific | Cat# 17502048 |
| NdeI | New England Biolabs | Cat# R0111S |
| Normal Goat Serum | Abcam | Cat# ab7481 |
| NuPAGE® 4-12% Bis-Tris gel | Invitrogen | Cat# NP0323BOX |
| NuPAGE MES SDS Running Buffer (20X) | Thermo Fisher Scientific | Cat# NP0002 |
| Orange G | Carl Roth | Cat# 0318.2 |
| Paraformaldehyde | Sigma-Aldrich | Cat# P6148 |
| pET23a | Novagen | Cat# 69745 |
| pET23a-ASC-tev-mCherry | Venegas et al., 2017 | N/A |
| Poly-L-Lysine Hybridomide | Sigma-Aldrich | Cat# P1524 |
| Protease/Phosphatase Inhibitor Cocktail (100X) | Cell Signaling Technology | Cat# 5872 |
| Protein LoBind Tubes | Eppendorf | 0030108116 |
| Q5 High Fidelity DNA polymerase | New England Biolabs | Cat# M0491S |
| SureBeadsTM Protein G Magnetic beads | Bio-Rad Laboratories | Cat# 1614023 |
| T4 DNA Ligase | New England Biolabs | Cat# M0202S |
| Trans-Blot® Turbo Mini Nitrocellulose Transfer Packs | Bio-Rad Laboratories | Cat# 1704158 |
| Critical Commercial Assays | ||
| Cytotoxicity Detection Kit (LDH) | Roche | Cat# 11644793001 |
| FAM-FLICA® Caspase-1 Assay Kit | ImmunoChemistry Technologies | Cat# 98 |
| Mouse IL-1 beta/IL-1 F2 DuoSet ELISA | R&D Systems | Cat# DY401 |
| Pierce™ BCA Protein Assay kit | Thermo Fischer Scientific | Cat# 23225 |
| Pierce™ LAL Chromogenic Endotoxin Quantitation Kit | Thermo Fischer Scientific | Cat# 88282 |
| QIAprep Spin Miniprep Kit | QIAGEN | Cat# 27104 |
| QIAquick Gel Extraction Kit | QIAGEN | Cat# 28704 |
| QIAquick PCR Purification Kit | QIAGEN | Cat# 28104 |
| XTT Cell Viability Kit | Cell Signaling Technology | Cat# 9095 |
| Experimental Models | ||
| NCTC clone 929 (L929 cells), strain: C3H/An | ATCC | Cat# CCL-1; RRID: CVCL_0462 |
| Primary microglia isolated from C57BL/6 mice | Charles River Laboratories | RRID: IMSR_JAX:000664 |
| Primary microglia isolated from C57BL/6 ASC−/− mice | Millenium Pharmaceuticals | N/A |
| E. coli BL21 (DE3), genotype: F– ompT hsdSB (rB–, mB–) gal dcm (DE3) | Merck | Cat#69450. |
| Software and Algorithms | ||
| FACSDIVA software | Becton Dickinson | N/A |
| Fiji ImageJ | Wayne Rusband | v2.0.0-rc-69/1.52n |
| FlowJo | FlowJo, LLC | V3.05470 |
| Graph Pad Prism | GraphPad Software Inc. | v7.0e |
| Image Studio | LI-COR Biosciences | v5.2 |
| NIS-Elements | Nikon | v4.0 |
| Other | ||
| Avanti J265 XP centrifuge | Beckmann Coulter | equipment |
| ÄKTAprime plus FPLC system | GE Healthcare | equipment |
| BD FACSCanto™ II | BD Biosciences | equipment |
| Eppendorf BioPhotometer D30 | Eppendorf | equipment |
| Infinite M200 Pro | TECAN | equipment |
| JA-25.50 Fixed-Angle Rotor | Beckmann Coulter | equipment |
| JEOL JEM-2200FS Field Emission Transmission Electron Microscope equipped with a CMOS-Camera | JEOL GmbH | equipment |
| JLA-8.1000 Fixed-Angle Rotor | Beckmann Coulter | equipment |
| JULABO 5 water bath | JULABO GmbH | equipment |
| Lab 850 pH-Meter | Schott Instruments | equipment |
| Multitron pro | Infors HT | equipment |
| NanoDrop 2000c Spectrophotometer | Thermo Scientific | equipment |
| Nikon Eclipse Ti Fluorescence Microscope | Nikon | equipment |
| ODYSSEY CLx Imaging System | LI-COR Biotechnology | equipment |
| Optima TLX ultracentrifuge | Beckmann Coulter | equipment |
| TLA-120.2 Fixed-Angle Rotor | Beckmann Coulter | equipment |
| Trans-Blot® Turbo™ Transfer System | Bio-Rad Laboratories | equipment |
| Vibra-Cell ultrasonic liquid processor | Sonics | equipment |