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. 2021 Dec 17;224(24):jeb242970. doi: 10.1242/jeb.242970

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — The expression of clock genes does not significantly vary during a 24-h period in the brain of sticklebacks in DD. Time-of-day-dependent relative expression of (A) bmal1a, (B) clock1b, (C) clock2, (D) per1b and (E) cry1b measured by qPCR in the brain of sticklebacks after 1 day in constant darkness (DD). The letter ‘a’ denotes the absence of significant difference between time points for the five genes (one-way ANOVA, P>0.05). The black line in the middle of each boxplot indicates the median and each dot represents an individual. Sample size is shown for each time point.