FIG. 7.

Sedimentation of Chl12, Rad24, and Rfc1 in sucrose density gradient centrifugation. Extracts were prepared from CHL12-HA RAD24-myc RFC1-FLAG (KSC1433) cells and separated by centrifugation in a 10 to 40% sucrose gradient for 16 h. The load on the gradient (L) and fractions (removed from the top of the gradient) were analyzed by immunoblotting using anti-FLAG, anti-HA, or anti-myc antibody. An F after a gene name indicates the addition of FLAG epitopes. Bovine serum albumin (4.5S) and thyroglobulin (16.5–19S) were separated simultaneously in an independent gradient as markers.