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. Author manuscript; available in PMC: 2022 Jan 5.
Published in final edited form as: Nat Cancer. 2021 Mar 11;2(3):327–339. doi: 10.1038/s43018-021-00179-8

Extended Data 4. Effect of TGFβ2 and BMP7 on signalling pathways (using ELK, p38 and p27 sensors) and MSCs in E0771 cancer cell growth.

Extended Data 4

a-d. T-HEp3 cells with ERK, p38 and p27 activity biosensors were reversed transfected with control siRNA or siRNAs for TGFBRIII and BMPRII followed by 24-hour treatments with TGFβ2 and BMP7. a. TGFBRIII and BMPRII mRNA levels 48 hours after transfection with the indicated siRNAs (n=3 independent experiments, mean, minimum and maximum, 2-tailed Mann–Whitney tests, p=0.01 (TGFbR3) and 0.002 (BMPR2)). b-d. Quantification of the T-HEp3-biosensors activity (n=3 independent experiments each, mean, minimum and maximum, 2-tailed Mann–Whitney tests, p=0.05). e. Percentage of E0771 cells in a single cell (p=0.002), doublet or cluster (p=0.005) state after co-culture with Control (passaged) or revitalized (r) MSCs (n=4 independent experiments, 4 wells per condition per experiment, mean and SEM, 2-tailed Mann–Whitney tests). f. qPCR of TGFβ1, TGFβ2 and BMP7 from Control and rMSCs (n=2). *p≤0.05, p-values indicated above.