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. 2022 Jan 5;16(1):e0010074. doi: 10.1371/journal.pntd.0010074

Fig 1. Experimental T. cruzi infection induces the expression of Wnt-5a in heart endothelial cells.

Fig 1

Lysates (20 μg) from WT or TSP1 KO MHEC challenged with T. cruzi at different time points were resolved by SDS-PAGE, blotted, and probed with antibodies against Wnt-5a antibody (A) WT MHEC and (B) TSP 1KO MHEC and developed as described. The blots were stripped, reprobed with antibodies against housekeeping GAPDH and developed with the corresponding IRDye conjugated secondary antibody. The developed blots were scanned using the infrared fluorescence detection Odyssey Imaging System. The normalized fold change of Wnt-5a at each time point was determined and plotted in the bar graph for WT MHEC (A, lower panel) and for TSP1 KO MHEC (B, lower panel), respectively. The bar graphs represent mean values ± SE from three independent biological replicates. The value of P< 0.05 was considered significant. *P< 0.05; **P< 0.01; ***P< 0.001.