Fig 3. Longer polyQ sequences decrease the transcriptional activity of AR.

(A,C) Luciferase reporter assays were performed with LNCaP cells and PC3 cells transfected with vectors expressing full-length AR containing 12, 20, 31 or 49 glutamines in the polyQ repeat. Luciferase activity was measured in the absence and presence of DHT, and the activities were normalized to co-transfected β-galactosidase activity. Bars show averages of values measured in three independent experiments performed under the same conditions and error bars show standard deviations. (B) Western blot showing the levels of AR in the LNCaP cells used for the assays shown in panel (A). Top: total AR using an anti-AR antibody; middle: overexpressed FLAG-AR using an anti-Flag antibody; and bottom: anti-β-actin used as a loading control.