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. 2022 Jan 5;18(1):e1010170. doi: 10.1371/journal.ppat.1010170

Fig 6. Sr0161 is not involved in the RplI mediated control of ExsA translation.

Fig 6

The protein levels of ExsA-Flag in mPAO1, ΔSr0161, ΔrplI and ΔrplIΔSr0161 containing pE267 (A); ΔSr0161 containing pE3331 and empty vector (pE2620) or pE2620-Sr0161 (B); mPAO1 and ΔrplI containing pE3331 and empty vector (pE2620) or pE2620-Sr0161 (C). Bacteria cultured overnight were diluted 1:50 into LB medium with 1 mM IPTG (B and C) or without IPTG (A), grown to an OD600 of 1.0 with (+) or without (-) 5 mM EGTA, and collected by centrifugation. Samples from equivalent numbers of bacterial cells were separated by SDS-PAGE and probed with an anti-Flag or an anti-RpoA antibody. The density of each band was determined with Image J. Relative densities represent the density of ExsA-Flag/density of RpoA with the respective first lane as 1. The data shown represent the results from three independent experiments.