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. 2022 Jan 5;18(1):e1010170. doi: 10.1371/journal.ppat.1010170

Fig 7. RplI interacts with exsA mRNA containing a 24 nt 5’ UTR but not a 12 nt 5’ UTR.

Fig 7

(A and B) Binding of RplI to the indicated RNAs was examined by EMSA. Increasing amounts of purified P. aeruginosa RplI-His were incubated with 167 nt/155 nt (A/B) exsA mRNA respective corresponding to the 24 nt/12 nt 5’ UTR and its first 143 nt mRNA on ice for 30 min. The mixtures were electrophoresed on an 8% native polyacrylamide gel, and the bands were visualized by staining with Gel-red for 10 min. (C) MST assay to test the binding capability of purified P. aeruginosa RplI-His to the indicated RNA (same as A, B) transcribed in vitro. The error bar represents the standard deviation from triplicate assays for each sample.