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. 2022 Jan 5;18(1):e1010170. doi: 10.1371/journal.ppat.1010170

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© 2022 Wang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — (A) Expression of ExoS in another PAO1 laboratory strain and PAK containing empty vector pUCP20 or pUCP20-rplI. (B and C) Expression of ExsA-Flag in PAK (B) and PAO1 (C) containing pE3330 or pE3331 with empty vector pE2620 or pE2620-rplI. Bacteria cultured overnight were diluted 1:50 into fresh LB medium, grown to an OD₆₀₀ of 1.0 with (+) or without (-) 5 mM EGTA and collected by centrifugation. Proteins from equivalent bacterial cells were separated by SDS-PAGE gels and probed with an antibody against ExoS/Flag or an anti-RpoA antibody. The density of each band was determined with Image J. Relative densities represent the density of ExoS or ExsA-Flag/density of RpoA with the respective first lane as 1. The data shown represent the results from three independent experiments.