Figure 1.
DRG neurons downregulate core synaptic transmission genes as they acquire axon growth competence
(A) Live-cell images of a pseudocolored (black) adult DRG neuron 0–36 h after plating. Scale bar, 200 μm.
(B) Axon growth velocity of (A). Values are plotted as mean ± SEM; ∗∗∗p < 0.0001, ∗∗p < 0.01 by one-way ANOVA followed by Tukey’s post hoc test; n = 3 independent experiments with 14, 17, and 19 neurons per experiment.
(C) Scheme of RRHO analysis: comparing whole-transcriptome changes in developing, regenerating, and cultured DRG.
(D) Heatmaps of –log10 p values comparing overlap between whole-genome gene-expression changes in PNL/Sham, E12.5/E17.5, and time in cell culture (6, 12, 24, and 36 h).
(E) Correlation analysis of gene-expression profiles of the 3 paradigms.
(F) Heatmap of differentially expressed genes in DRG at E12.5, E17.5, and adult with PNL, Sham, or after 6–36 h in culture from RNA-seq with GO term “synapse” downregulated from 6 to 36 h (338 genes; FDR = 0.001; FDR-adjusted p < 1 × 10−5).
(G) Sunburst plot insets of SynGO-enrichment analyses showing the locations synaptic vesicle and presynaptic active zone and the function synaptic vesicle exocytosis enriched for in synapse-related genes downregulated in adult DRG neurons after 36 h in cell culture. Color code denotes the –log10 Q-value score.
See also Figures S1–S3 and Tables S1 and S2.