TABLE 1.

Primer sequences used for direct raw sewage reverse-transcriptase quantitative loop-mediated isothermal amplification (RT qLAMP)

Gene	Primer	Sequence 5′→3′	No. of bases
Lamb et al,16 2020, 63°C; Color Genomics,5 2020, 65°C
ORF 1a	F3	TCCAGATGAGGATGAAGAAGA	21
ORF 1a	B3	AGTCTGAACAACTGGTGTAAG	21
ORF 1a	FIP	AGAGCAGCAGAAGTGGCACAGGTGATTGTGAAGAAGAAGAG	41
ORF 1a	BIP	TCAACCTGAAGAAGAGCAAGAACTGATTGTCCTCACTGCC	40
ORF 1a	LF	CTCATATTGAGTTGATGGCTCA	22
ORF 1a	LB	ACAAACTGTTGGTCAACAAGAC	22
Broughton et al,17,18 2020, 62°C; Color Genomics,5 2020, 65°C
N	F3	AACACAAGCTTTCGGCAG	18
N	B3	GAAATTTGGATCTTTGTCATCC	22
N	FIP	TGCGGCCAATGTTTGTAATCAGCCAAGGAAATTTTGGGGAC	41
N	BIP	CGCATTGGCATGGAAGTCACTTTGATGGCACCTGTGTAG	39
N	LF	TTCCTTGTCTGATTAGTTC	19
N	LB	ACCTTCGGGAACGTGGTT	18
Broughton et al,17,18 2020, 62°C; Color Genomics,5 2020, 65°C
E	F3	CCGACGACGACTACTAGC	18
E	B3	AGAGTAAACGTAAAAAGAAGGTT	23
E	FIP	ACCTGTCTCTTCCGAAACGAATTTGTAAGCACAAGCTGATG	41
E	BIP	CTAGCCATCCTTACTGCGCTACTCACGTTAACAATATTGCA	41
E	LF	TCGATTGTGTGCGTACTGC	19
E	LB	TGAGTACATAAGTTCGTAC	19

Abbreviations: B3, backward outer; F3, forward outer; BIP, backward inner primer; FIP, forward inner primer; LB, loop backward; LF, loop forward.