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. 2021 Sep;32(3):98–101. doi: 10.7171/jbt.21-3203-004

FIGURE 2.

FIGURE 2

Testing of the S6970 primer set and molecular beacons on synthetic SARS-CoV-2 RNA. Fluorescence was monitored over time for LAMP isothermal amplification using the S6970 primer set alone (A) or S6970 multiplexed with N2 primer (B) for Twist Australia/VIC01/2020 (Twist WT) and Twist England/205041766/2020 (Twist B.1.1.7). The amount of synthetic SARS-CoV-2 RNA in each reaction was varied from 10,000 to 39 copies (indicated by line color) or water control (black). All fluorescence is given as relative fluorescence units (RFUs) divided by 1000.