FIGURE 1.

Two comparisons of phenol red against LAMPShade Violet for detection of amplification products in reverse transcription loop-mediated isothermal amplification (RT-LAMP). (a) A comparison of color change fidelity and consistency before and after incubation in 4 varied saliva samples as a function of sodium hydroxide (NaOH). (b) A comparison of the sensitivity of the commercial phenol red RT-LAMP mix and our in-house reaction mix with LAMPShade Violet judged by their ability to detect 20 total copies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA.