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Optimizing the binding and elution times for magnetic bead-based purification using carboxylated beads with 700-mM sodium chloride (NaCl) and 14% polyethylene glycol (PEG), as measured by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA recovery through N1 qPCR from contrived saliva samples. (a) The effect of binding time on RNA recovery, with a 1-minute elution. (b) The effect of elution time on RNA recovery, with a 5-minute binding time.
