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. 2021 Sep;32(3):102–113. doi: 10.7171/jbt.21-3203-005

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Several features of SalivaBeads purification. (a) A comparison of preincubation sample color uniformity in 12 samples, with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and actin reactions side by side. Direct input of inactivated saliva in a commercial phenol red-based reaction is compared to a SalivaBeads-purified RNA input into our LAMPShade Violet-based reaction. (b) A comparison of SARS-CoV-2 RNA recovered from contrived saliva samples by N1 quantitative polymerase chain reaction (qPCR), measured by number of copies recovered by qPCR divided by number of copies contrived into saliva. (c) A visual comparison of debris bound to magnetic beads when isolating beads by magnetic rack (left) or a magnetic stick (right). (d) A comparison of sensitivity between SalivaBeads purified via magnetic rack (left) and magnetic stick (right), by RT-LAMP with LAMPShade Violet.