TABLE 1.

Primer sequences and concentrations used to produce a 5× primer solution

Concentration	Component	Sequence
1 μM	E1-F3	TGAGTACGAACTTATGTACTCAT
1 μM	E1-B3	TTCAGATTTTTAACACGAGAGT
8 μM	E1-FIP	ACCACGAAAGCAAGAAAAAGAAGTTCGTTTCGGAAGAGACAG
8 μM	E1-BIP	TTGCTAGTTACACTAGCCATCCTTAGGTTTTACAAGACTCACGT
2 μM	E1-LB	GCGCTTCGATTGTGTGCGT
2 μM	E1-LF	CGCTATTAACTATTAACG
1 μM	Orf1a-HMS-F3	CGGTGGACAAATTGTCAC
1 μM	Orf1a-HMS-B3	CTTCTCTGGATTTAACACACTT
8 μM	Orf1a-HMS-LF	TTACAAGCTTAAAGAATGTCTGAACACT
8 μM	Orf1a-HMS-LB	TTGAATTTAGGTGAAACATTTGTCACG
2 μM	Orf1a-HMSe-FIP	TCAGCACACAAAGCCAAAAATTTATTTTTCTGTGCAAAGGAAATTAAGGAG
2 μM	Orf1a-HMSe-BIP	TATTGGTGGAGCTAAACTTAAAGCCTTTTCTGTACAATCCCTTTGAGTG