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. 2022 Jan 5;8(1):eabj1793. doi: 10.1126/sciadv.abj1793

Fig. 2. Translation efficiency of miR-21-5p–ON switches in HeLa and 293FT cells.

Fig. 2.

(A) Fold change of the translation efficiency of the miR-21-5p–ON switch. “miR-21” indicates an ON switch having an antisense sequence for miR-21-5p, and “shuffle” indicates an ON switch having a shuffled antisense sequence for miR-21-5p. All values are shown as the ratios of the translation efficiency (EGFP/iRFP670) in HeLa to 293FT cells transfected with mRNA having extra sequences [495 nucleotides (nt), 1250 nt, or 30 CAG repeats] downstream of the poly(A) tail. Error bars represent means ± SD (n = 3), and data of each biological replicate are shown as a point. ****P < 0.0005 (Welch’s t test). (B) Ratiometric images of cells cotransfected with iRFP670 mRNA and miR-21-5p–ON switch, which has the miR-21-5p antisense sequence or shuffled sequence. iRFP670 mRNA was used as an internal control. The EGFP intensity at each pixel in a cell was divided by the iRFP670 intensity. Red ratiometric pseudo-color indicates a higher ratio (translation efficiency) of EGFP to iRFP670. Scale bar, 200 μm.