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. 2022 Jan 5;8(1):eabj1793. doi: 10.1126/sciadv.abj1793

Fig. 7. Purification from mixed cells by miRNA-ON and -OFF switches.

Fig. 7.

(A) Microscopic images of cocultured HeLa and 293FT cells 3 days after the transfection. HeLa cells stably expressing hmAG1-M9 and 293FT cells stably expressing iRFP670-M9 were used to distinguish each cell line. Top images are bright-field images, and bottom images are merged images of hmAG1 and iRFP670. Green shows the hmAG1 signal, and red shows the iRFP670 signal. Scale bar, 200 μm. (B) Representative 2D flow cytometry plots. The squares indicate the gates of each cell. (C) Percentage of each cell. The number of each cell type was counted inside the squares in (B), and the percentage of each cell line was calculated. Error bars represent the means ± SD (n = 3), and data of each biological replicate is shown as a point. ****P < 0.0005.