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. 2021 Dec 20;10:e75583. doi: 10.7554/eLife.75583

Figure 4. FoSir5 affects ATP production during gemination in Fusarium oxysporum.

(A–B) Western blot analysis showed the dynamic changes of FoDLAT K148 (A) and histone H3K18 (B) crotonylation during germination using the indicated antibodies. Numbers below the blots represent the relative abundance of FoDLAT-K148cr or H3K18cr. Anti-GFP or anti-H3 immunoblotting was used to show equal loading, respectively. (C–D) Pyruvate dehydrogenase complex (PDC) activity (C) and acetyl-CoA production (D) in F. oxysporum during germination were determined. (E) Expression profile of the aerobic respiration-related genes during the germination process. (F) Relative enrichment of the immunoprecipitated promoter regions in aerobic respiration-related genes during germination determined using anti-GFP antibody in the FoSir5-GFP strain driven by the native promoter. The fold enrichment was normalized to the input and internal control gene (β-tubulin). (G) ATP content of F. oxysporum during germination. (H) Effect of FoSir5 on the ATP content of the indicated strains, as determined in germinating conidia at 8 hr post incubation (h.p.r.). The presence of different letters (A–H) above the mean values of three replicates indicates a significant difference between different samples (p < 0.05, ANOVA).

Figure 4—source data 1. FoSir5 affects ATP production during gemination in Fusarium oxysporum.
(A–B) Western blot analysis showed the dynamic changes of FoDLAT K148 (A) and histone H3K18 (B) crotonylation during germination using the indicated antibodies. Numbers below the blots represent the relative abundance of FoDLAT-K148cr or H3K18cr. Anti-GFP or anti-H3 immunoblotting was used to show equal loading, respectively. (C–D) Pyruvate dehydrogenase complex (PDC) activity (C) and acetyl-CoA production (D) in F. oxysporum during germination were determined. (E) Expression profile of the aerobic respiration-related genes during the germination process. (F) Relative enrichment of the immunoprecipitated promoter regions in aerobic respiration-related genes during germination determined using anti-GFP antibody in the FoSir5-GFP strain driven by the native promoter. The fold enrichment was normalized to the input and internal control gene (β-tubulin). (G) ATP content of F. oxysporum during germination. (H) Effect of FoSir5 on the ATP content of the indicated strains, as determined in germinating conidia at 8 hr post incubation (h.p.r.). The presence of different letters (A–H) above the mean values of three replicates indicates a significant difference between different samples (p < 0.05, ANOVA). (The red arrow indicates the original SDS–PAGE gels that were cropped for this panel.)

Figure 4.

Figure 4—figure supplement 1. The ATP content of ΔFoSir5 mutant (A) and OE-1 strain (B) during germinating process.

Figure 4—figure supplement 1.

The presence of different letters above the mean values of three replicates indicates a significant difference between different samples (p < 0.05, ANOVA).
Figure 4—figure supplement 2. Impact of FoSir5 on the virulence of Fusarium oxysporum.

Figure 4—figure supplement 2.

(A) Pathogenicity of the indicated strains in tomato after 8 days of incubation. (B) Quantification of the disease indexes of the indicated strains. (C) Quantitative real-time PCR (qRT-PCR) analysis of F. oxysporum EF-1α transcript levels in tomato plants harvested 14 days after infection with the indicated strains. The expression of tomato RCE1, a constitutively expressed gene, was used as a control for the use of equal amounts of RNA for RT-PCR. The letters (B and C) above the mean values of three replicates indicate significant differences between different strains (p < 0.05, ANOVA).