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Identification of the RSP2 protein. (A) Genetic map of the R27 plasmid region where both the rsp and rsp2 (R0055) genes were mapped (dark gray arrows). (B) Comparison of the Big domains of the RSP and RSP2 proteins. (C) Detection of the RSP2 protein in the cell-free secretome of the SL1344(R27) strain. Arrows point to the bands corresponding to the RSP and RSP2 proteins, the latter of which was confirmed by LC-MS/MS analysis.
