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. 2022 Jan 5;7(1):e00978-21. doi: 10.1128/msphere.00978-21

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Copyright © 2022 Hüttener et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Immunodetection of the RSP2-Flag protein in different cellular compartments. (A) Coomassie blue staining of the different cellular fractions (periplasm, cytoplasm, outer membrane [OM], and inner membrane [IM]) obtained from strains SL11344 wt, SL1344(R27 RSP-Flag) (used as a control), and SL1344(R27 RSP2-Flag). (B) Immunodetection of the RSP-Flag and RSP2-Flag proteins in the periplasm, cytoplasm, and outer and inner membrane fractions of strains SL11344 wt, SL1344(R27 RSP-Flag), and SL1344(R27 RSP2-Flag), respectively. Arrows point to RSP-Flag (control) and RSP2-Flag proteins, respectively. (C) Immunodetection of the RSP2-Flag protein in the cell-free secretome of strains SL11344 wt and SL1344(R27 RSP2-Flag) grown at 25°C until OD₆₀₀ of 2.0. The arrow points to RSP2-Flag protein.