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. 2022 Jan 5;7(1):e00978-21. doi: 10.1128/msphere.00978-21

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Copyright © 2022 Hüttener et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — The RSP and RSP2 proteins bind to the conjugative pilus encoded by the R27 plasmid. (A and C) Immunogold electron microscopy of cells from strains SL1344 ΔfliC ΔfljB (R27) (A) and SL1344 ΔfliC ΔfljB (R27 ΔtrhH) (B) using polyclonal anti-RSP antibodies and goat anti-rabbit IgG conjugated to 12-nm gold particles. Arrows point to the RSP protein associated with the conjugative pilus. (C and D) Immunogold electron microscopy of cells from strains SL1344 ΔfliC ΔfljB (R27 RSP2-Flag) (C) and SL1344 ΔfliC ΔfljB (R27 RSP2-Flag ΔtrhH) (D) using anti-Flag monoclonal antibodies and goat anti-mouse IgG conjugated to 12-nm gold particles. Arrows point to the RSP2-Flag protein associated with the conjugative pilus. Bars represent 1 μm (A, B, and D) and 2 μm (C).