FIG. 5.

AMF1 stimulates transcriptional activation by p53. (A) Transient transactivation in U2OS cells. A total of 500 ng of p53-dependent luciferase reporters PG13 or pWAF1-luciferase (19) was cotransfected into U2OS cells with 0, 1, or 4 μg of AMF1-expressing vector pDB327. At 32 h after transfection, luciferase activities were measured and are presented as the increase in activation over that of the reporter alone. Each sample was analyzed in triplicate, and standard deviations are shown (error bars). (B) Transient transactivation in Saos-2 cells. The same procedure as that for panel A was used, except that 50 ng of vector pC53SN was cotransfected to provide wild-type p53 activity in the cell.