Table 1. The oligonucleotide primers used for amplification of genes encoding efflux pumps.
Target gene Primer | Primer Sequence (5–3) | Amplicon size (bp) |
---|---|---|
qacZ | F-ATATTGCTTACGCAAGTTGG | 156 |
R-AGTGTGATGATCCGAATGTG | ||
qacG | F-CAACAGAAATAATCGGAACT | 275 |
R-TACATTTAAGAGCACTACA | ||
qacJ | F-GGCCAACATTAGGCACACTTA | 232 |
R-TGACTTGATCCAAAAACGTTAAGA | ||
qacΔΕ | F-GGCTTTACTAAGCTTGCCCC | 127 |
R-AGCCCCATACCTACAAAGCC | ||
oqxA | F-GGGGAAACATTCACCTGAC | 407 |
R-ACGGGAGACGAGGTTGGTA | ||
oqxB | F-CTCGGCTCGTCTTATGTCAAT | 567 |
R-TTACAGGCCCAGGCCCATC | ||
emeA | F-AGCCCAAGCGAAAAGCGGTTT | 128 |
R-CCATCGCTTTCGGACGTTCA | ||
efrA | F-TTGGCTTTATGACGCCAGT | 224 |
R-ATGCGCGTATTACCCGCAA | ||
efrB | F-TAGTGATGATGTTCTTAATCAA | 211 |
R-ATTGACTTGTTTAAAGCCTTCA | ||
qacA/B | F-GCAGAAAGTGCAGAGTTCG | 361 |
R-CCAGTCCAATCATGCCTG | ||
qacC | F-AAACAATGCAACACCTACCACT | 157 |
R-AACGAAACTACGCCGACTATG | ||
smr (qacC + qacD) | F-GCCATAAGTACTGAAGTTATTGGA | 195 |
R-GACTACGGTTGTTAAGACTAAACCT |