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. 2021 Dec 29;2021:5177488. doi: 10.1155/2021/5177488

Figure 2.

Figure 2

circFAT1 does not affect PDLSCs proliferation. (a) Cell proliferation capability influenced by circFAT1 was detected at 450 nm with CCK-8 assay. (I): CCK-8 results showed that circFAT1 had no significant effect on the proliferation of PDLSCs; (II): EdU assay further confirmed that circFAT1 had no significant effect on the proliferation of PDLSCs. (b) EdU-positive PDLSCs influenced by circFAT1 were detected by EdU kit (scale bar: 200 μm). EdU (red), Hoechst333 (blue). (c) PCR gel electrophoresis verified that the primers were located at a single band of 449 kb. The cyclization site of circFAT1 was determined by plasmid vector construction. (d) RNA nucleocytoplasmic isolation experiments verified that circFAT1 was mainly located in the cytoplasm of cells. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. EdU: 5-ethynyl-2-deoxyuridine.