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. 2021 Dec 29;2021:5177488. doi: 10.1155/2021/5177488

Figure 5.

Figure 5

Overexpression of miR-4781-3p decreases osteoblastic differentiation tendency of PDLSCs. (a)–(c) The results of Western blot and RT-qPCR showed SMAD5 and osteogenic markers (RUNX2, OSX, COL1A, and COL3A) were downregulated in miR-4781-3p mimic transfected PDLSCs (mimics group). In addition, RT-qPCR results showed that the expression of circFAT1 was downregulated, and miR-4781-3p was upregulated in the mimics group. (d, e) Overexpression of miR-4781-3p reduced the ALP activity of PDLSCs 7 days after osteogenic induction. ARS showed the mineralization of PDLSCs was decreased significantly in the mimic group after osteogenic induction for 14 days. (f) Immunofluorescence experiments confirmed the decreased expression of ALP in the mimics group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.