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. 2021 Dec 29;2021:5177488. doi: 10.1155/2021/5177488

Figure 6.

Figure 6

Knockdown of miR-4781-3p increases osteoblastic differentiation tendency of PDLSCs. (a)–(c) The results of Western blot and RT-qPCR showed SMAD5 and osteogenic markers (RUNX2, OSX, COL1A, and COL3A) were upregulated in the miR-4781-3p inhibitor transfected PDLSCs (inhibitor group). In addition, RT-qPCR results showed that the expression of circFAT1 was upregulated, and miR-4781-3p was downregulated in the inhibitor group. (d, e) Knockdown of miR-4781-3p increased the ALP activity of PDLSCs 7 days after osteogenic induction. ARS showed the mineralization of PDLSCs was added significantly in the inhibitor group after osteogenic induction for 14 days. (f) Immunofluorescence experiments confirmed the increased expression of ALP in the inhibitor group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.