TABLE 1.
Transforming properties of cell lines in the presence and absence of PAC1
| Mutant used to transform K2F6 | PAC1 | Morphologya | Growth in soft agar (no. of colonies/103 cells)b |
|---|---|---|---|
| None | − | Flat | 0 |
| + | Flat | 0 | |
| MEK(218D,222D) | − | Spindle | 42 |
| + | Flat | 0.43 | |
| RafΔN | − | Spindle | 22 |
| + | Flat | 14 | |
| Ras(12V) | − | Spindle | 147 |
| + | Spindle | 147 | |
| Ras(12V,37G) | − | Flat | 2.0 |
| + | Flat | 0.72 |
a
Morphology of cells was observed in DMEM supplemented with 10% calf serum.
b
Determination of growth in soft agar was performed as outlined previously (7). The results are averages for triplicate samples and are representative of at least three independent experiments.