TABLE 4.
Metastasis formation by cell lines with modified RalGEF-Ral pathways
| Cell line and pathway(s) | PAC1 | Avg no. of tumors/mousea | % of mice with tumors | Range of tumor no. |
|---|---|---|---|---|
| 3T3, MEK(218D,222D) | NA | 6 | 60 | 0–20 |
| 3T3 MEK(218D,222D) + Ral(28N) | NA | 9 | 75 | 0–20 |
| K2F6, RalGDS-CAAX | − | 7 | 100 | 2–20 |
| + | 3 | 100 | 1–5 | |
| 3T3, Ral(23V) | NA | 0 | 0 | 0 |
| 3T3, Ral(39L) | NA | 0 | 0 | 0 |
a
Athymic mice were injected intravenously with 105 cells as described in Materials and Methods. The average number of macroscopic tumors per mouse was determined 6 weeks following injection. At least 20 mice were injected with each cell line.