FIG. 5.

Northern blot and Western blot analysis of brevican and tenascin-C genes and proteins from NCCre-targeted animals. (A) Northern blot hybridizations of brevican and tenascin-C genes derived from wild-type (+/+), heterozygous (+/−), and homozygous (−/−) mice. GAPDH is a loading control. (B and C) Western blot of D10 and D30 soluble brain extract (B) and total homogenate (C) of wild-type (+/+) and mutant mice (−/−) detected by antisera against brevican (B) and tenascin-C (C), respectively. (B) Samples were treated with (+) and without (−) chondroitinase ABC (ch'se).