Fig. 2.

4T1 Screening Results and Validation. (A) Mean nuclear area from well images was used as a primary analysis for G2/M stall, with hits more than 1 SD from the mean (above dotted line). 1 of 5 plates of the drug library is shown. (B) Nuclear intensity histograms were generated from the same images and used to further narrow in on G2/M arrest hits (above dotted line, indicating 3 SD). (C) Mean nuclear area and nuclear intensity showed a loose correlation with false positives for nuclear size in the upper left quadrant, and hits for both parameters in the highlighted quadrant. After identifying hits, they were validated by dosing at a range of concentrations with an automated printer and generating histograms (D), cell cycle profiles (E), cell viability (F), and cell cycle index (G). *D–G are from G2/M hit proflavine hemisulfate.