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. 2001 Sep;21(17):6044–6055. doi: 10.1128/MCB.21.17.6044-6055.2001

FIG. 3.

FIG. 3

Subcellular localization of KNT analyzed by confocal microscopy. Double labeling of KNT-eGFP (A, D, E, and F; green)-transfected 3T3 cells with anticalreticulin (B and C; red), anti-gm130 (D; red) and anti-α-tubulin (E and G; red) antibody. The merged image (B) shows the colocalization of KNT-eGFP (A) with the ER (C). KNT-eGFP does not colocalize with cis-Golgi cisternae (D) and microtubules (E). (F and G) In heavily overexpressing cells, KNT-eGFP can be detected in the periphery. Magnification, ×1,000.