FIG. 4.
Unaltered stationary localization of membrane organelles in EF lacking KNT as shown by immunolabeling of untreated control EF (A to E) and kntΔ/Δ EF (F to J). (A and F) Anti-α-tubulin indirect immunofluorescence staining. (C and H) Mitochondrial labeling with Mitotracker. (B and G) Merged image of mitochondria and microtubules showing the correct colocalization of mitochondria with the microtubules in wild-type and null mutant cells. Anti-gm130 (D and I) and anticalreticulin (E and J) indirect immunofluorescence labeling indicates the unimpaired assembly of cis-Golgi and ER in the absence of KNT. Magnification, ×1,000.