FIG. 5.

Intact anterograde and retrograde lysosome transport in knt^Δ/Δ and knt^+/+ EF. (A to L) Lysosomes were directly labeled with FITC-conjugated anti-LAMP-1 antibody in wild-type (A, B, E, F, I, and J) and knt^Δ/Δ (C, D, G, H, K, and L) EF. Labeling of mitochondria with Mitotracker (B, D, F, H, J, and L) in red was used to control cellular integrity. (A to D) Stationary localization of lysosomes in EF incubated in R. (E to H) Perinuclear clustering of lysosomes was caused by alkaline treatment (BR) in wild-type and knt^Δ/Δ cells. (I to L) In cells of both genotypes, acidification of the culture medium (AR) resulted in transport of lysosomes to the cell periphery. Magnification, ×900.