TABLE 1.
Synchronization of lysosome localization by pharmacological treatment
| Treatment medium and genotype | Subcellular localization of lysosomes (avg deviation)a
|
||
|---|---|---|---|
| % Clustered | % Dispersed | % Peripheral | |
| R | |||
| +/+ | 4.5 (3.5) | 95.5 (3.5) | 0 (0) |
| Δ/Δ | 5.8 (3.3) | 92.0 (3.0) | 2.2 (1.8) |
| F/F | 2.5 (2.5) | 88.5 (6.5) | 9.0 (9.0) |
| BR | |||
| +/+ | 85.5 (0.5) | 14.5 (0.5) | 0 (0) |
| Δ/Δ | 85.8 (5.4) | 14.0 (5.0) | 0.2 (0.4) |
| F/F | 82.5 (4.5) | 17.5 (4.5) | 0 (0) |
| AR | |||
| +/+ | 0 (0) | 11.5 (3.5) | 88.5 (3.5) |
| Δ/Δ | 0 (0) | 23.0 (1.0) | 77.0 (1.0) |
| F/F | 0 (0) | 13.5 (9.5) | 86.5 (9.5) |
a
One hundred cells per sample were scored for the subcellular localization of their lysosomes blindly with respect to the prior treatment and genotype of the cell samples. Percentages were calculated from two independent experiments, and the average deviations are given in parentheses. The Δ/Δ values were calculated from two independent Δ/Δ EF lines; a third Δ/Δ EF line that was included in only one of the experiments showed similar results. For experimental procedures, see Fig. 5 and Materials and Methods.