FIG. 3.

Suppression of rpa190-G728D by RPB6. (A) Strain NOY265 (41) was transformed by URA3⁺ plasmids bearing the yeast (2μm RPB6) or human (2μm Hs6) RPB6 genes or the centromeric URA3 plasmid bearing the yeast RPB6 gene (CEN RPB6). The control strain was NOY265 transformed with a void plasmid (pFL44L [vector]). Cells were streaked on YPD and incubated for 5 days at 37°C. (B) A local alignment between the largest subunits of yeast (Rpa190p, Rpb1p, Rpc160p), human Rpb1p (Rpb1p Hs), and archaeal (A′ Sa) RNA polymerases. The black triangle indicates the position of the rpa190-G728D mutation. ∗, identity; +, similarity.