Fig. 3. Dendritic cell and regulatory T-cell numbers, retinaldehyde dehydrogenase activity/mRNA expression, and regulatory B-cell-derived cytokines are preserved in IL-10−/−/β7−/− mice.
a Percentage of CD11c+ DC and CD4+CD25+FOXP3+ regulatory T cells within the colonic LP of the indicated mouse strains, as determined by flow cytometry (noninflamed (N/I), inflamed (I), as determined by histology). Events gated on live, single cells. b Aldefluor conversion within the colonic LP DC fraction isolated from the indicated strains. c Relative mRNA expression of RALDH1, RALDH2, and RALDH3 and d of regulatory cytokines and subunits within colonic tissues of indicated mouse strains. Expression of the respective genes was normalized to GAPDH expression. Each data point represents an individual mouse. Data are presented as mean ± S.D from n > 6 mice in each dataset. Statistical significance determined using ANOVA.