Extended Data Fig. 4. Rescue assays.
a, Western blots of wild-type or CLIMP63 knockout cells transfected with empty vector or the indicated CLIMP63 constructs. b, Representative fluorescence images and quantifications of CLIMP63 knockout cells transfected with empty vector or the indicated CLIMP63 constructs. c, Western blots of wild-type or CLIMP63 knockout cells transfected with empty vector or the indicated CLIMP63 constructs. CLIMP63-3SE indicates S3E, S17E and S19E triple mutation of CLIMP63. d, Quantifications of ER morphology in wild-type or CLIMP63 KO cells expressing the indicated CLIMP63 constructs. n = 43, 31, 46 cells (left to right). e, f, Western blots and representative images of wild-type or p180 KO cells expressing the indicated p180 constructs. g–i, Western blots and representative images of wild-type or KTN1 KO cells expressing the indicated KTN1 constructs. j–l, p180 knockout U2OS cells were transfected with mApple, p180s-mApple, or p180s mutants lacking kinesin-binding domain (KBD), then imaged and quantified for ER morphology. Scale bars, 10 μm. n = 82, 53, 53 cells for both (k) and (l) (left to right). Data are mean ± s.d. P values shown on top, two-tailed t-tests. See Supplementary Information for uncropped western blots.