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. 2021 Aug 21;15(1):74–83. doi: 10.1038/s41385-021-00441-3

Fig. 3. PTPN2-knockdown macrophages fail to induce IL-4Rα upon treatment with IL-6.

Fig. 3

THP-1 cells expressing non-targeting control (shCtr) or PTPN2-specific (shPTPN2) shRNA were differentiated into macrophages and treated for 24 h (A + D) or the indicated time (B + C) with 20 ng/ml IL-6. Depicted are a mRNA expression of IL4RA normalized to GAPDH and untreated shCtr cells, b representative Western blot images for IL-4Rα and β-actin, c respective densitometry analysis, and d representative flow cytometry histograms for surface IL-4Ra expression. Gated on life, single cells. ** = p < 0.01 relative to untreated control, ## = p < 0.05 relative to IL-6 treated shCtr cells, ANOVA with Dunnett’s multiple comparisons test. Representative results from one out of three independent experiments with n = 5 (A + B) or n = 3 (C + D) independent replicates.