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. 2021 Aug 21;15(1):74–83. doi: 10.1038/s41385-021-00441-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–c THP-1 cells expressing non-targeting control (shCtr) or PTPN2-specific (shPTPN2) shRNA were differentiated into macrophages and treated with IL-6 in combination with or without recombinant sIL-6Rα for 30 min (A) or 24 h (B + C). Depicted are a representative Western blot pictures for the indicated proteins, b mRNA expression levels of IL4A normalized to GAPDH and untreated control cells, c flow cytometry histograms for surface expression of IL-4Rα (CD124). d, e: THP-1 cells expressing non-targeting control (shCtr) or PTPN2-specific (shPTPN2) shRNA were differentiated into macrophages and treated with IL-6 in combination with recombinant sIL-6Rα for 6 h prior to stimulation with IL-4 for 30 min (D) or 24 h (e, f). The graphs show d representative Western blot images and e mRNA expression of MRC1 (encoding CD206), IL10, and TGFB normalized to GAPDH and untreated control cells. * = p < 0.05, ** = p < 0.01, *** = p < 0.001, ANOVA with Dunnett’s multiple comparisons test. Representative results from three independent experiments with 5 independent replicates (n = 5).